In Silico Analysis of structural and Functional Impact of Non Synonymous SNPs in DC-Sign Receptor Gene

Abstract

Background: Cd209 encodes very important trans membrane receptor DC-SIGN in DC and macrophages to recognize and bind with carbohydrate ligands present on surface of pathogen. Structural alterations of this receptor can greatly influence its functions. nsSNPs is important group of SNPs family which brings structural alteration in protein.

Objective: In this study, we sorted out SNPs present in coding region of CD20 and also evaluated their impact of nsSNPs on structure of protein through computational tools.

Methodology: Genomic data has been retrieved from 1000 Genome project and then sorted out by using computational tools including SNpEff, PolyPhen-2, SIFT, PMUT, MutPred, I-Mutant and PROVEAN.

Results: Out of 177 SNPs of coding region, 30 were sorted out as nsSNPs. Among 30 nsSNPs, only 12 nsSNPs have been predicted deleterious.

Conclusion: All pathogenic nsSNPs have been found in Neck and C-lectin damain of receptor which is involved in recognition and binding of ligands. It can be concluded that these nsSNPs can play their role in genetic susceptibility of individuals toward infectious diseases.